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guinea pig anti vglut 1  (Synaptic Systems)


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    Structured Review

    Synaptic Systems guinea pig anti vglut 1
    Guinea Pig Anti Vglut 1, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 95/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guinea+pig+anti-vglut-1/131+002/pm42443391-346-32-36
    Average 95 stars, based on 171 article reviews
    guinea pig anti vglut 1 - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: MT3-MMP Promotes Excitatory Synapse Formation by Promoting Nogo-66 Receptor Ectodomain Shedding
    Article Snippet: For immunofluorescence, the following antibodies were used: mouse anti-PSD95 (11,000; Millipore), rabbit anti-synapsin-1 (1:1000; Millipore), mouse anti-PSD95 (1:200; NeuroMab), guinea pig anti-VGLUT-1 (1:400; Synaptic Systems), mouse anti-Myc (1:500; Sigma-Aldrich), and goat anti-human IgG Fc (1:500; Jackson ImmunoResearch).

    Article Title: N-cadherin and Neuroligins Cooperate to Regulate Synapse Formation in Hippocampal Cultures
    Article Snippet: The primary antibodies used were guinea pig anti-VGlut-1 (Synaptic Systems), mouse antiPSD-95 (Affinity BioReagents), rabbit anti-N-cadherin (generous gift from Dr. David Colman), and rabbit anti-NL2 (generous gift from Dr. Ann-Marie Craig).

    Article Title: Synaptic Remodeling of Entorhinal Input Contributes to an Aberrant Hippocampal Network in Temporal Lobe Epilepsy.
    Article Snippet: For immunofluorescence staining, free-floating sagittal sections were pretreated with 0.25% TritonX-100 in 1% bovine serum D ow nloaded from https://academ ic.oup.com /cercor/article/27/3/2348/3056317 by guest on 31 M ay 2024 albumin for 1 h. Subsequently, serial tissue sections were either directly incubated with Streptavidin-Alexafluor 546 (1:1000, Molecular Probes) for 2.5 h to detect BDA or with the following primary antibodies at 4 °C overnight: guinea pig anti-NeuN (1:500, Synaptic Systems, Göttingen, Germany), guinea pig anti-vGlut-1 (1:1000, Synaptic Systems), or rabbit anti-PSD-95 (1:1000, Abcam, Cambridge, UK).

    Expressing:

    Article Title: MT3-MMP Promotes Excitatory Synapse Formation by Promoting Nogo-66 Receptor Ectodomain Shedding
    Article Snippet: For immunofluorescence, the following antibodies were used: mouse anti-PSD95 (11,000; Millipore), rabbit anti-synapsin-1 (1:1000; Millipore), mouse anti-PSD95 (1:200; NeuroMab), guinea pig anti-VGLUT-1 (1:400; Synaptic Systems), mouse anti-Myc (1:500; Sigma-Aldrich), and goat anti-human IgG Fc (1:500; Jackson ImmunoResearch).

    Article Title: N-cadherin and Neuroligins Cooperate to Regulate Synapse Formation in Hippocampal Cultures
    Article Snippet: The primary antibodies used were guinea pig anti-VGlut-1 (Synaptic Systems), mouse antiPSD-95 (Affinity BioReagents), rabbit anti-N-cadherin (generous gift from Dr. David Colman), and rabbit anti-NL2 (generous gift from Dr. Ann-Marie Craig).

    Article Title: Synaptic Remodeling of Entorhinal Input Contributes to an Aberrant Hippocampal Network in Temporal Lobe Epilepsy.
    Article Snippet: For immunofluorescence staining, free-floating sagittal sections were pretreated with 0.25% TritonX-100 in 1% bovine serum D ow nloaded from https://academ ic.oup.com /cercor/article/27/3/2348/3056317 by guest on 31 M ay 2024 albumin for 1 h. Subsequently, serial tissue sections were either directly incubated with Streptavidin-Alexafluor 546 (1:1000, Molecular Probes) for 2.5 h to detect BDA or with the following primary antibodies at 4 °C overnight: guinea pig anti-NeuN (1:500, Synaptic Systems, Göttingen, Germany), guinea pig anti-vGlut-1 (1:1000, Synaptic Systems), or rabbit anti-PSD-95 (1:1000, Abcam, Cambridge, UK).

    Staining:

    Article Title: MT3-MMP Promotes Excitatory Synapse Formation by Promoting Nogo-66 Receptor Ectodomain Shedding
    Article Snippet: For immunofluorescence, the following antibodies were used: mouse anti-PSD95 (11,000; Millipore), rabbit anti-synapsin-1 (1:1000; Millipore), mouse anti-PSD95 (1:200; NeuroMab), guinea pig anti-VGLUT-1 (1:400; Synaptic Systems), mouse anti-Myc (1:500; Sigma-Aldrich), and goat anti-human IgG Fc (1:500; Jackson ImmunoResearch).

    Article Title: N-cadherin and Neuroligins Cooperate to Regulate Synapse Formation in Hippocampal Cultures
    Article Snippet: The primary antibodies used were guinea pig anti-VGlut-1 (Synaptic Systems), mouse antiPSD-95 (Affinity BioReagents), rabbit anti-N-cadherin (generous gift from Dr. David Colman), and rabbit anti-NL2 (generous gift from Dr. Ann-Marie Craig).

    Article Title: Synaptic Remodeling of Entorhinal Input Contributes to an Aberrant Hippocampal Network in Temporal Lobe Epilepsy.
    Article Snippet: For immunofluorescence staining, free-floating sagittal sections were pretreated with 0.25% TritonX-100 in 1% bovine serum D ow nloaded from https://academ ic.oup.com /cercor/article/27/3/2348/3056317 by guest on 31 M ay 2024 albumin for 1 h. Subsequently, serial tissue sections were either directly incubated with Streptavidin-Alexafluor 546 (1:1000, Molecular Probes) for 2.5 h to detect BDA or with the following primary antibodies at 4 °C overnight: guinea pig anti-NeuN (1:500, Synaptic Systems, Göttingen, Germany), guinea pig anti-vGlut-1 (1:1000, Synaptic Systems), or rabbit anti-PSD-95 (1:1000, Abcam, Cambridge, UK).

    Plasmid Preparation:

    Article Title: MT3-MMP Promotes Excitatory Synapse Formation by Promoting Nogo-66 Receptor Ectodomain Shedding
    Article Snippet: For immunofluorescence, the following antibodies were used: mouse anti-PSD95 (11,000; Millipore), rabbit anti-synapsin-1 (1:1000; Millipore), mouse anti-PSD95 (1:200; NeuroMab), guinea pig anti-VGLUT-1 (1:400; Synaptic Systems), mouse anti-Myc (1:500; Sigma-Aldrich), and goat anti-human IgG Fc (1:500; Jackson ImmunoResearch).

    Article Title: N-cadherin and Neuroligins Cooperate to Regulate Synapse Formation in Hippocampal Cultures
    Article Snippet: The primary antibodies used were guinea pig anti-VGlut-1 (Synaptic Systems), mouse antiPSD-95 (Affinity BioReagents), rabbit anti-N-cadherin (generous gift from Dr. David Colman), and rabbit anti-NL2 (generous gift from Dr. Ann-Marie Craig).

    Article Title: Synaptic Remodeling of Entorhinal Input Contributes to an Aberrant Hippocampal Network in Temporal Lobe Epilepsy.
    Article Snippet: For immunofluorescence staining, free-floating sagittal sections were pretreated with 0.25% TritonX-100 in 1% bovine serum D ow nloaded from https://academ ic.oup.com /cercor/article/27/3/2348/3056317 by guest on 31 M ay 2024 albumin for 1 h. Subsequently, serial tissue sections were either directly incubated with Streptavidin-Alexafluor 546 (1:1000, Molecular Probes) for 2.5 h to detect BDA or with the following primary antibodies at 4 °C overnight: guinea pig anti-NeuN (1:500, Synaptic Systems, Göttingen, Germany), guinea pig anti-vGlut-1 (1:1000, Synaptic Systems), or rabbit anti-PSD-95 (1:1000, Abcam, Cambridge, UK).

    Recombinant:

    Article Title: MT3-MMP Promotes Excitatory Synapse Formation by Promoting Nogo-66 Receptor Ectodomain Shedding
    Article Snippet: For immunofluorescence, the following antibodies were used: mouse anti-PSD95 (11,000; Millipore), rabbit anti-synapsin-1 (1:1000; Millipore), mouse anti-PSD95 (1:200; NeuroMab), guinea pig anti-VGLUT-1 (1:400; Synaptic Systems), mouse anti-Myc (1:500; Sigma-Aldrich), and goat anti-human IgG Fc (1:500; Jackson ImmunoResearch).

    Article Title: N-cadherin and Neuroligins Cooperate to Regulate Synapse Formation in Hippocampal Cultures
    Article Snippet: The primary antibodies used were guinea pig anti-VGlut-1 (Synaptic Systems), mouse antiPSD-95 (Affinity BioReagents), rabbit anti-N-cadherin (generous gift from Dr. David Colman), and rabbit anti-NL2 (generous gift from Dr. Ann-Marie Craig).

    Article Title: Synaptic Remodeling of Entorhinal Input Contributes to an Aberrant Hippocampal Network in Temporal Lobe Epilepsy.
    Article Snippet: For immunofluorescence staining, free-floating sagittal sections were pretreated with 0.25% TritonX-100 in 1% bovine serum D ow nloaded from https://academ ic.oup.com /cercor/article/27/3/2348/3056317 by guest on 31 M ay 2024 albumin for 1 h. Subsequently, serial tissue sections were either directly incubated with Streptavidin-Alexafluor 546 (1:1000, Molecular Probes) for 2.5 h to detect BDA or with the following primary antibodies at 4 °C overnight: guinea pig anti-NeuN (1:500, Synaptic Systems, Göttingen, Germany), guinea pig anti-vGlut-1 (1:1000, Synaptic Systems), or rabbit anti-PSD-95 (1:1000, Abcam, Cambridge, UK).

    Generated:

    Article Title: MT3-MMP Promotes Excitatory Synapse Formation by Promoting Nogo-66 Receptor Ectodomain Shedding
    Article Snippet: For immunofluorescence, the following antibodies were used: mouse anti-PSD95 (11,000; Millipore), rabbit anti-synapsin-1 (1:1000; Millipore), mouse anti-PSD95 (1:200; NeuroMab), guinea pig anti-VGLUT-1 (1:400; Synaptic Systems), mouse anti-Myc (1:500; Sigma-Aldrich), and goat anti-human IgG Fc (1:500; Jackson ImmunoResearch).

    Article Title: N-cadherin and Neuroligins Cooperate to Regulate Synapse Formation in Hippocampal Cultures
    Article Snippet: The primary antibodies used were guinea pig anti-VGlut-1 (Synaptic Systems), mouse antiPSD-95 (Affinity BioReagents), rabbit anti-N-cadherin (generous gift from Dr. David Colman), and rabbit anti-NL2 (generous gift from Dr. Ann-Marie Craig).

    Article Title: Synaptic Remodeling of Entorhinal Input Contributes to an Aberrant Hippocampal Network in Temporal Lobe Epilepsy.
    Article Snippet: For immunofluorescence staining, free-floating sagittal sections were pretreated with 0.25% TritonX-100 in 1% bovine serum D ow nloaded from https://academ ic.oup.com /cercor/article/27/3/2348/3056317 by guest on 31 M ay 2024 albumin for 1 h. Subsequently, serial tissue sections were either directly incubated with Streptavidin-Alexafluor 546 (1:1000, Molecular Probes) for 2.5 h to detect BDA or with the following primary antibodies at 4 °C overnight: guinea pig anti-NeuN (1:500, Synaptic Systems, Göttingen, Germany), guinea pig anti-vGlut-1 (1:1000, Synaptic Systems), or rabbit anti-PSD-95 (1:1000, Abcam, Cambridge, UK).

    Infection:

    Article Title: MT3-MMP Promotes Excitatory Synapse Formation by Promoting Nogo-66 Receptor Ectodomain Shedding
    Article Snippet: For immunofluorescence, the following antibodies were used: mouse anti-PSD95 (11,000; Millipore), rabbit anti-synapsin-1 (1:1000; Millipore), mouse anti-PSD95 (1:200; NeuroMab), guinea pig anti-VGLUT-1 (1:400; Synaptic Systems), mouse anti-Myc (1:500; Sigma-Aldrich), and goat anti-human IgG Fc (1:500; Jackson ImmunoResearch).

    Article Title: N-cadherin and Neuroligins Cooperate to Regulate Synapse Formation in Hippocampal Cultures
    Article Snippet: The primary antibodies used were guinea pig anti-VGlut-1 (Synaptic Systems), mouse antiPSD-95 (Affinity BioReagents), rabbit anti-N-cadherin (generous gift from Dr. David Colman), and rabbit anti-NL2 (generous gift from Dr. Ann-Marie Craig).

    Article Title: Synaptic Remodeling of Entorhinal Input Contributes to an Aberrant Hippocampal Network in Temporal Lobe Epilepsy.
    Article Snippet: For immunofluorescence staining, free-floating sagittal sections were pretreated with 0.25% TritonX-100 in 1% bovine serum D ow nloaded from https://academ ic.oup.com /cercor/article/27/3/2348/3056317 by guest on 31 M ay 2024 albumin for 1 h. Subsequently, serial tissue sections were either directly incubated with Streptavidin-Alexafluor 546 (1:1000, Molecular Probes) for 2.5 h to detect BDA or with the following primary antibodies at 4 °C overnight: guinea pig anti-NeuN (1:500, Synaptic Systems, Göttingen, Germany), guinea pig anti-vGlut-1 (1:1000, Synaptic Systems), or rabbit anti-PSD-95 (1:1000, Abcam, Cambridge, UK).

    Incubation:

    Article Title: MT3-MMP Promotes Excitatory Synapse Formation by Promoting Nogo-66 Receptor Ectodomain Shedding
    Article Snippet: For immunofluorescence, the following antibodies were used: mouse anti-PSD95 (11,000; Millipore), rabbit anti-synapsin-1 (1:1000; Millipore), mouse anti-PSD95 (1:200; NeuroMab), guinea pig anti-VGLUT-1 (1:400; Synaptic Systems), mouse anti-Myc (1:500; Sigma-Aldrich), and goat anti-human IgG Fc (1:500; Jackson ImmunoResearch).

    Article Title: N-cadherin and Neuroligins Cooperate to Regulate Synapse Formation in Hippocampal Cultures
    Article Snippet: The primary antibodies used were guinea pig anti-VGlut-1 (Synaptic Systems), mouse antiPSD-95 (Affinity BioReagents), rabbit anti-N-cadherin (generous gift from Dr. David Colman), and rabbit anti-NL2 (generous gift from Dr. Ann-Marie Craig).

    Article Title: Synaptic Remodeling of Entorhinal Input Contributes to an Aberrant Hippocampal Network in Temporal Lobe Epilepsy.
    Article Snippet: For immunofluorescence staining, free-floating sagittal sections were pretreated with 0.25% TritonX-100 in 1% bovine serum D ow nloaded from https://academ ic.oup.com /cercor/article/27/3/2348/3056317 by guest on 31 M ay 2024 albumin for 1 h. Subsequently, serial tissue sections were either directly incubated with Streptavidin-Alexafluor 546 (1:1000, Molecular Probes) for 2.5 h to detect BDA or with the following primary antibodies at 4 °C overnight: guinea pig anti-NeuN (1:500, Synaptic Systems, Göttingen, Germany), guinea pig anti-vGlut-1 (1:1000, Synaptic Systems), or rabbit anti-PSD-95 (1:1000, Abcam, Cambridge, UK).



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    Image Search Results


    Double labelling for vGluT-1 and vGluT-2 . (a) In the molecular layer, puncta immulabelled for vGluT-1 (green) are densely distributed in the neuropil; puncta immulabelled for vGluT-2 (red) are localized in the inner zone of the layer, in anatomical relation to body and dendrite trunks of Purkinje neurons. (b) In the granular layer, puncta double immunolabelled for vGluT-1 and vGluT-2 (yellow), and puncta immunoreactive for vGluT-1 or VGluT-2 are present. Scale bar: 10 μm.

    Journal: BMC Neuroscience

    Article Title: VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex

    doi: 10.1186/1471-2202-12-118

    Figure Lengend Snippet: Double labelling for vGluT-1 and vGluT-2 . (a) In the molecular layer, puncta immulabelled for vGluT-1 (green) are densely distributed in the neuropil; puncta immulabelled for vGluT-2 (red) are localized in the inner zone of the layer, in anatomical relation to body and dendrite trunks of Purkinje neurons. (b) In the granular layer, puncta double immunolabelled for vGluT-1 and vGluT-2 (yellow), and puncta immunoreactive for vGluT-1 or VGluT-2 are present. Scale bar: 10 μm.

    Article Snippet: The following commercial antibodies were used: 1. anti-vGluT-1 polyclonal antibody, raised in guinea pig against a synthetic linear peptide from rat vGluT-1 (Millipore, Billerica, MA, USA) [ , ]; 2. anti-vGluT-2 polyclonal antibody, raised in rabbit against a strep-tag fusion protein of rat vGluT-2 (Synaptic System, Goettingen, Germany) [ , ]; 3. (a) anti-GAD monoclonal antibody, raised in mouse against human GAD-65-GST fusion protein (Stressgen, Victoria, BC, Canada), and (b) anti-GAD polyclonal antibody, raised in rabbit against a synthetic peptide corresponding to the rat GAD-65 C-terminus residues 572-585 (Chemicon), which react to both GAD-65 and GAD-67 [ ]; 4. (a) anti-VAMP monoclonal antibody, raised in mouse against crude synaptic immunoprecipitate (human) (Millipore), and (b) anti-VAMP polyclonal antibody, raised in rabbit against a synthetic peptide based on rat VAMP-2 (Stressgen), which react to VAMP-2 [ ]; 5. anti-SNAP-25 monoclonal antibody, raised in against crude synaptic immunoprecipitate (human), which reacts with both SNAP-25A and SNAP-25B (Chemicon) [ ]; 6. anti-syntaxin monoclonal antibody, raised in mouse against clone HCP-1, which reacts to syntaxin-1 (ABCAM, Cambridge, UK) [ ].

    Techniques:

    Double labelling for vGluT-1 and VAMP-2, VGluT-1 and SNAP-25A/B, VGluT-1 and syntaxin-1 . (a, b, c; g, h, i; m, n, o): molecular/Purkinje layer; (d, e, f; j, k, l; p, q, r): granular layer. In the molecular/Purkinje and granular layers, numerous punctate elements which display co-localization of vGluT-1 with VAMP-2 (c, f) or SNAP-25A/B (i, l) or syntaxin-1 (o, r) are seen. However, in all layers, a number of vGluT-1-immunoreactive puncta appear unlabelled for VAMP-2, SNAP-25A/B and syntaxin-1. Scale bars: a, b, c, d, e, f, m, n, o, p, q, r: 20 μm; g, h, i, j, k, l: 50 μm.

    Journal: BMC Neuroscience

    Article Title: VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex

    doi: 10.1186/1471-2202-12-118

    Figure Lengend Snippet: Double labelling for vGluT-1 and VAMP-2, VGluT-1 and SNAP-25A/B, VGluT-1 and syntaxin-1 . (a, b, c; g, h, i; m, n, o): molecular/Purkinje layer; (d, e, f; j, k, l; p, q, r): granular layer. In the molecular/Purkinje and granular layers, numerous punctate elements which display co-localization of vGluT-1 with VAMP-2 (c, f) or SNAP-25A/B (i, l) or syntaxin-1 (o, r) are seen. However, in all layers, a number of vGluT-1-immunoreactive puncta appear unlabelled for VAMP-2, SNAP-25A/B and syntaxin-1. Scale bars: a, b, c, d, e, f, m, n, o, p, q, r: 20 μm; g, h, i, j, k, l: 50 μm.

    Article Snippet: The following commercial antibodies were used: 1. anti-vGluT-1 polyclonal antibody, raised in guinea pig against a synthetic linear peptide from rat vGluT-1 (Millipore, Billerica, MA, USA) [ , ]; 2. anti-vGluT-2 polyclonal antibody, raised in rabbit against a strep-tag fusion protein of rat vGluT-2 (Synaptic System, Goettingen, Germany) [ , ]; 3. (a) anti-GAD monoclonal antibody, raised in mouse against human GAD-65-GST fusion protein (Stressgen, Victoria, BC, Canada), and (b) anti-GAD polyclonal antibody, raised in rabbit against a synthetic peptide corresponding to the rat GAD-65 C-terminus residues 572-585 (Chemicon), which react to both GAD-65 and GAD-67 [ ]; 4. (a) anti-VAMP monoclonal antibody, raised in mouse against crude synaptic immunoprecipitate (human) (Millipore), and (b) anti-VAMP polyclonal antibody, raised in rabbit against a synthetic peptide based on rat VAMP-2 (Stressgen), which react to VAMP-2 [ ]; 5. anti-SNAP-25 monoclonal antibody, raised in against crude synaptic immunoprecipitate (human), which reacts with both SNAP-25A and SNAP-25B (Chemicon) [ ]; 6. anti-syntaxin monoclonal antibody, raised in mouse against clone HCP-1, which reacts to syntaxin-1 (ABCAM, Cambridge, UK) [ ].

    Techniques:

    Double labelling for vGluT-1 and syntaxin-1 . In (a) the molecular layer and (b) granular layer, double labelled punctate elements, putative axon terminals belonging to parallel fibres and mossy fibres, respectively, are seen. In both the layers, subpopulation of terminals positive only for vGluT-1 are present. Scale bar: 10 μm.

    Journal: BMC Neuroscience

    Article Title: VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex

    doi: 10.1186/1471-2202-12-118

    Figure Lengend Snippet: Double labelling for vGluT-1 and syntaxin-1 . In (a) the molecular layer and (b) granular layer, double labelled punctate elements, putative axon terminals belonging to parallel fibres and mossy fibres, respectively, are seen. In both the layers, subpopulation of terminals positive only for vGluT-1 are present. Scale bar: 10 μm.

    Article Snippet: The following commercial antibodies were used: 1. anti-vGluT-1 polyclonal antibody, raised in guinea pig against a synthetic linear peptide from rat vGluT-1 (Millipore, Billerica, MA, USA) [ , ]; 2. anti-vGluT-2 polyclonal antibody, raised in rabbit against a strep-tag fusion protein of rat vGluT-2 (Synaptic System, Goettingen, Germany) [ , ]; 3. (a) anti-GAD monoclonal antibody, raised in mouse against human GAD-65-GST fusion protein (Stressgen, Victoria, BC, Canada), and (b) anti-GAD polyclonal antibody, raised in rabbit against a synthetic peptide corresponding to the rat GAD-65 C-terminus residues 572-585 (Chemicon), which react to both GAD-65 and GAD-67 [ ]; 4. (a) anti-VAMP monoclonal antibody, raised in mouse against crude synaptic immunoprecipitate (human) (Millipore), and (b) anti-VAMP polyclonal antibody, raised in rabbit against a synthetic peptide based on rat VAMP-2 (Stressgen), which react to VAMP-2 [ ]; 5. anti-SNAP-25 monoclonal antibody, raised in against crude synaptic immunoprecipitate (human), which reacts with both SNAP-25A and SNAP-25B (Chemicon) [ ]; 6. anti-syntaxin monoclonal antibody, raised in mouse against clone HCP-1, which reacts to syntaxin-1 (ABCAM, Cambridge, UK) [ ].

    Techniques: